Journal: Acta Pharmaceutica Sinica. B

Article Title: Hyperimonates A and B, a pair of unprecedented polyprenylated acylphloroglucinols from Hypericum monogynum : Structural elucidation, total synthesis, and lipid-lowering activity

doi: 10.1016/j.apsb.2025.10.008

Figure Lengend Snippet: Effects of compounds 1 and 2 on lipid accumulation in OA/Fr-induced HepG2 cells. (A–D) Effects of compounds 1 and 2 on the accumulation of TG in OA/Fr-induced HepG2 cells and their toxicity. (E, F) Lipid accumulation was observed for com. 1 with ORO staining. Results are expressed as the mean ± standard deviations (SD) ( n = 3), scale bar = 100 μm ## P < 0.01 and ### P < 0.001 compared with the control group; ∗∗ P < 0.01 and ∗∗∗ P < 0.001 compared with the model group.

Article Snippet: The human liver-derived cell line HepG2 was obtained from the Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Staining, Control

Journal: Acta Pharmaceutica Sinica. B

Article Title: Hyperimonates A and B, a pair of unprecedented polyprenylated acylphloroglucinols from Hypericum monogynum : Structural elucidation, total synthesis, and lipid-lowering activity

doi: 10.1016/j.apsb.2025.10.008

Figure Lengend Snippet: The mechanistic study of compound 1 on the lipid accumulation in HepG2 cells. (A–F) Proteomic study when treated with 1 . (A) Principal component analysis (PCA). (B) Model (M) vs . control (CN) differentially expressed proteins (DEPs) volcano diagram. (C) Com. 1 vs . M DEPs volcano diagram. (D) DEPs between M and CN group, as well as com. 1 and M. group. (E) Clustering heat map of differential protein expression in the three groups. (F) KEGG pathway enrichment analysis.

Article Snippet: The human liver-derived cell line HepG2 was obtained from the Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Control, Expressing

Journal: Acta Pharmaceutica Sinica. B

Article Title: Hyperimonates A and B, a pair of unprecedented polyprenylated acylphloroglucinols from Hypericum monogynum : Structural elucidation, total synthesis, and lipid-lowering activity

doi: 10.1016/j.apsb.2025.10.008

Figure Lengend Snippet: Effect of compound 1 on the expression levels of proteins in OA/Fro-induced HepG2 cells. (A, G) The expression levels of proteins were analyzed by Western blot in OA/Fro-induced HepG2 cells after treatment with compound 1 for 48 h. (B–F, H–M) Schematic diagram showing the mechanism of the protective effect of compound 1 on lipid metabolism disorder. Results are expressed as the mean ± SD ( n = 3). # P < 0.05, ## P < 0.01 and ### P < 0.001 compared with the control group; ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 compared with the model group.

Article Snippet: The human liver-derived cell line HepG2 was obtained from the Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Expressing, Western Blot, Control

Journal: Antioxidants

Article Title: Myrobalan Fruit Extracts Modulate Immunobiochemical Pathways In Vitro

doi: 10.3390/antiox14030350

Figure Lengend Snippet: Effects of BB3 and the fruit extracts on cell viability. HepG2 cells were treated with vehicle (EtOH) or BB3 or myrobalan extracts (12.5–200 μg/mL) for 24 ( A ), 48 ( B ), and 72 h ( C ). Mean values ± S.E.M. of four independent experiments run in duplicates (* p < 0.05 and ** p < 0.005; compared to untreated cells) are shown.

Article Snippet: The human liver cancer-derived cell line HepG2 (RRID:CVCL_0027; DSMZ, Germany) was cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco—Thermo Fisher Scientific, Vienna, Austria) supplemented with 10% ( v / v ) heat-inactivated FCS.

Techniques:

Journal: Antioxidants

Article Title: Myrobalan Fruit Extracts Modulate Immunobiochemical Pathways In Vitro

doi: 10.3390/antiox14030350

Figure Lengend Snippet: Measurement of intracellular ROS. Inhibition of peroxyl-radical (AAPH)-induced formation of ROS in HepG2 cells pretreated with increasing concentrations of BB3 or the myrobalan extracts (12.5–200 μg/mL). The mean percentages of DCF fluorescence, as a measure of ROS formation, are shown in relation to the AAPH-treated EtOH vehicle control (set to 100%). Mean values ± S.E.M. of three independent experiments run in quadruplicates (** p < 0.01; compared to AAPH-treated cells) are shown.

Article Snippet: The human liver cancer-derived cell line HepG2 (RRID:CVCL_0027; DSMZ, Germany) was cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco—Thermo Fisher Scientific, Vienna, Austria) supplemented with 10% ( v / v ) heat-inactivated FCS.

Techniques: Inhibition, Fluorescence, Control

Journal: Antioxidants

Article Title: Myrobalan Fruit Extracts Modulate Immunobiochemical Pathways In Vitro

doi: 10.3390/antiox14030350

Figure Lengend Snippet: Influence of plant extracts on ß-lactamase activity, which was taken as a measure for ARE-dependent gene expression, in CellSensor ® ARE-bla HepG2 cells (expressed as n-fold to the corresponding control solvent). Shown are the mean values ± S.E.M. of three independent experiments with four parallels per concentration. (* p < 0.05 and ** p < 0.01; compared to vehicle-treated control cells).

Article Snippet: The human liver cancer-derived cell line HepG2 (RRID:CVCL_0027; DSMZ, Germany) was cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco—Thermo Fisher Scientific, Vienna, Austria) supplemented with 10% ( v / v ) heat-inactivated FCS.

Techniques: Activity Assay, Gene Expression, Control, Solvent, Concentration Assay